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Biochem Biophys Res Commun. 2001 Aug 10;286(1):184-8.

Functional abnormalities in protein tyrosine phosphatase epsilon-deficient macrophages.

Author information

1
Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, A'Beckett Street, Melbourne, Victoria 8006, Australia.

Abstract

Protein tyrosine phosphatase epsilon (PTP epsilon)-deficient mice were generated by targeted deletion of exons 3, 4, and 5 of the Ptpre gene. Mice homozygous for this deletion (Ptpre(Delta3-5)) were fertile, bred and developed normally and exhibited no overt phenotype. However, closer examination of the function of macrophages from these mice revealed a defect in the regulation of the respiratory burst. While bacterial lipopolysaccharide (LPS) or tumour necrosis factor alpha (TNFalpha) were able to prime bone marrow-derived macrophages (BMM) from wild type (Ptpre(+)) macrophages for an enhanced respiratory burst, they were unable to do so in macrophages from PTP epsilon-deficient mice. PTP epsilon-deficient BMM also had abnormalities in cytokine production with a reduced ability to produce TNFalpha and enhanced IL-10 production in response to challenge with LPS. These findings suggest an important role for PTP epsilon in the control of macrophage function.

PMID:
11485326
DOI:
10.1006/bbrc.2001.5360
[Indexed for MEDLINE]

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