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Nat Cell Biol. 2001 Aug;3(8):E175-8.

DNA microarrays and beyond: completing the journey from tissue to cell.

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1
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA.

Erratum in

  • Nat Cell Biol 2001 Oct;3(10):943.

Abstract

For the cell biologist, identifying changes in gene expression using DNA microarrays is just the start of a long journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.

PMID:
11483971
DOI:
10.1038/35087108
[Indexed for MEDLINE]
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