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Fertil Steril. 2001 Aug;76(2):400-2.

Successful pregnancies and deliveries after a simple vitrification protocol for day 3 human embryos.

Author information

1
California Reproductive Laboratories, San Francisco, California 94109, USA. idanasouri@aol.com

Abstract

OBJECTIVE:

To evaluate the efficacy and efficiency of freezing cleaved human embryos through vitrification.

DESIGN:

Clinical study of vitrification of human embryos.

SETTING:

Assisted reproductive technology centers.

PATIENT(S):

Thirty-six patients undergoing IVF-ICSI treatment whose surplus embryos were frozen.

INTERVENTION(S):

Two hundred fifteen surplus embryos vitrified, subsequently thawed, and transferred in natural or controlled cycles.

MAIN OUTCOME MEASURE(S):

Embryo survival rate after thawing and resultant patient pregnancy rate.

RESULT(S):

From the 215 vitrified and thawed embryos, 106 survived, with an overall embryo survival rate of 49.3%. The survival rate was higher when embryos were vitrified at the eight-cell stage compared with at the six to seven-cell and six-cell stages (79.2%, 39.7%, and 21.1%, respectively). On average, 2.9 +/- 1.2 embryos per patient were transferred, resulting in 11 pregnancies (30.5%), with an implantation rate of 10.4% per embryo transferred.

CONCLUSION(S):

Ultrarapid embryo freezing by vitrification of eight-cell stage embryos is a reliable method, as evidenced by high rates of embryo survival and pregnancy, making it a superior alternative to the conventional slow-cooling method.

PMID:
11476797
[Indexed for MEDLINE]

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