Format

Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr A. 2001 Jul 6;921(2):109-19.

Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support.

Author information

  • 1Department of Biochemical Engineering, Tianjin University, China.

Abstract

A poly(vinyl alcohol)-based magnetic gel entrapping Fe3O4 colloids has been prepared by an emulsification-crosslinking method. The gel was modified with Cibacron blue 3GA, and thus a magnetic affinity support was produced. The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin obeyed the Henry's law. Uptake kinetics of the two proteins was investigated and analyzed with a pore diffusion model and a homogeneous diffusion model. Experimental results showed that the magnetic affinity gel had magnetic responsiveness and favorable properties in protein adsorption, and was mechanically and chemically stable.

PMID:
11471795
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center