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[Rapid detection of drug resistant HIV -1 to reverse transcriptase and protease inhibitors in HIV-infected patients receiving highly active antiretroviral therapies].

Author information

1
Children's Memorial Institute for Education and Research, Northwestern University Medical School, Chicago, Illinois, USA.

Abstract

OBJECTIVE:

To develop and validate a rapid and sensitive method that can be used to detect emergence of HIV-1 drug resistant viruses in HIV-infected patients receiving highly active antiretroviral therapies (HAART).

METHODS:

HIV-1 viral RNA was extracted from plasma and amplified by nested RT-PCR. Nucleotide sequences of the reverse transcriptase (RT) and protease (PR) genes were determined by automated DNA sequencing.

RESULTS:

RT and PR sequences can be reliably determined with a minimum 2,000 copies/ml of viral RNA in plasma. Emergence of drug resistant viruses can he detected as soon as it reached to > 25% of the total viral populations. By using this method, drug-resistant viral populations were found to correlate with rebound of viral RNA level after prolonged antiretroviral therapies in HIV-infected children.

CONCLUSIONS:

This rapid and sensitive HIV-1 genotyping method can be used to predict and confirm emergence of HIV-1 drug resistance in HIV-infected patients receiving HAART.

PMID:
11471017
[Indexed for MEDLINE]

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