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J Bacteriol. 2001 Aug;183(16):4900-4.

Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily.

Author information

1
Department of Biological Chemistry, Institute of Molecular Biology, University of Copenhagen, 1307 Copenhagen K, Denmark.

Abstract

The specificity of XapB permease was compared with that of the known nucleoside transporters NupG and NupC. XapB-mediated xanthosine uptake is abolished by 2,4-dinitrophenol and exhibits saturation kinetics with an apparent K(m) of 136 microM. A 12-transmembrane-segment model was confirmed by translational fusions to alkaline phosphatase and the alpha fragment of beta-galactosidase.

PMID:
11466294
PMCID:
PMC99545
DOI:
10.1128/JB.183.16.4900-4904.2001
[Indexed for MEDLINE]
Free PMC Article

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