Format

Send to

Choose Destination
EMBO Rep. 2001 Jul;2(7):598-603. Epub 2001 Jul 3.

A genetic look at the active site of RNA polymerase III.

Author information

1
Service de Biochimie & Génétique Moléculaire, CEA/Saclay. Bât. 142, F-91191 Gif/Yvette, France.

Abstract

rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements at the two large subunits of the enzyme. The mutated motifs are conserved in RNA polymerases I and II and, for some of them, in the bacterial enzyme, thus delineating key elements of the active site in eukaryotic RNA polymerases.

PMID:
11454743
PMCID:
PMC1083950
DOI:
10.1093/embo-reports/kve136
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center