Comparison of SeroMP IgA with four other commercial assays for serodiagnosis of Mycoplasma pneumoniae pneumonia

Diagn Microbiol Infect Dis. 2001 May-Jun;40(1-2):21-5. doi: 10.1016/s0732-8893(01)00250-4.

Abstract

Acute phase serum samples from 23 patients with Mycoplasma pneumoniae pneumonia were examined for specific antibodies with microparticle agglutination (MAG)-assay, complement fixation (CF)-test, IgM-specific ELISA, and Immunocard-based IgM-EIA. A novel 3-h IgA-ELISA (SeroMP-IgA) was tested to assess its diagnostic value. For MAG, CF, IgM-ELISA, card-based IgM-EIA, and IgA-ELISA positive results were obtained in 87%, 87%, 91%, 87%, and 100%, respectively. Overall concordance was 78%. Specificity testing of 46 healthy blood donors revealed an optimal diagnostic cut-off range of 22-30 IgA-ELISA binding units (BU)/mL resulting in 91%-100% specificity and 100%-96% sensitivity for serologic diagnosis of acute M. pneumoniae infection.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Bacterial / blood*
  • Child
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immunoglobulin A / blood*
  • Middle Aged
  • Mycoplasma pneumoniae / immunology*
  • Pneumonia, Mycoplasma / diagnosis*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Species Specificity

Substances

  • Antibodies, Bacterial
  • Immunoglobulin A
  • Reagent Kits, Diagnostic