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Life Sci. 2001 Jun 8;69(3):271-80.

Impaired repair activity of a truncated DNA polymerase beta protein.

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Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, OH 44195, USA.


DNA polymerase beta (polbeta) is an essential enzyme for gap filling synthesis in damaged DNA template involved in base excision repair pathway. A truncated polbeta protein is expressed in primary colorectal and breast adenocarcinomas. To determine a possible alteration in the functions of the enzyme, a human cell line named HeLapolbetadelta expressing the truncated form of polbeta has been established. These cells revealed a significantly reduced level of repair activity evaluated by gap filling synthesis and polbeta activity. More importantly, the HeLapolbetadelta cells are hypersensitive to MNNG, a DNA alkylating agent. It appears from the responses that the gap filling synthesis of WT cells, a HeLa cell line overexpressing wild-type polbeta protein, was inhibited by HeLapolbetadelta protein.

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