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Dev Biol. 2001 Jul 15;235(2):343-50.

Loss of Xist imprinting in diploid parthenogenetic preimplantation embryos.

Author information

1
X Inactivation Group, MRC Clinical Sciences Centre, ICSM, Hammersmith Hospital, DuCane Rd, London W12 ONN, United Kingdom.

Abstract

We have analysed Xist expression patterns in parthenogenetic and control fertilised preimplantation embryos by using RNA FISH. In normal XX embryos, maternally derived Xist alleles are repressed throughout preimplantation development. Paternal alleles are expressed as early as the 2-cell stage. In parthenogenetic embryos, we observed Xist RNA expression and accumulation from the morula stage onwards, indicating loss of maternal imprinting. In the majority of cells, expression was from a single allele, indicating that X chromosome counting occurs to establish appropriate monoallelic Xist expression. We discuss these data in the context of models for regulation of imprinted and random X inactivation.

PMID:
11437441
DOI:
10.1006/dbio.2001.0295
[Indexed for MEDLINE]
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