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Nat Cell Biol. 2001 Jul;3(7):643-9.

Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour.

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1
Department of Genetics, Wellcome/CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK.

Abstract

The XMAP215/ch-TOG/Msps family of microtubule-associated proteins (MAPs) promote microtubule growth in vitro and are concentrated at centrosomes in vivo. We show here that Msps (mini-spindles protein) interacts with the centrosomal protein D-TACC, and that this interaction strongly influences microtubule behaviour in Drosophila embryos. If D-TACC levels are reduced, Msps does not concentrate at the centrosomes efficiently and the centrosomal microtubules appear to be destabilized. If D-TACC levels are increased, both D-TACC and Msps accumulate around the centrosomes/spindle poles, and the centrosomal microtubules appear to be stabilized. We show that the interaction between D-TACC and Msps is evolutionarily conserved. We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome.

PMID:
11433296
DOI:
10.1038/35083033
[Indexed for MEDLINE]
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