Abstract
The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans- acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cells, Cultured
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Chimera
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Crosses, Genetic
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DNA Methylation
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Female
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Fibroblasts / cytology
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Fibroblasts / physiology
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Gene Expression Regulation*
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Genomic Imprinting*
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Genomic Library
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Insulin-Like Growth Factor II / genetics*
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Promoter Regions, Genetic
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Recombinant Proteins / metabolism
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Repressor Proteins / metabolism*
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Restriction Mapping
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Reverse Transcriptase Polymerase Chain Reaction
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Transfection
Substances
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GCFC2 protein, human
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Recombinant Proteins
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Repressor Proteins
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Insulin-Like Growth Factor II