An upstream repressor element plays a role in Igf2 imprinting

EMBO J. 2001 Jul 2;20(13):3518-25. doi: 10.1093/emboj/20.13.3518.

Abstract

The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans- acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cells, Cultured
  • Chimera
  • Crosses, Genetic
  • DNA Methylation
  • Female
  • Fibroblasts / cytology
  • Fibroblasts / physiology
  • Gene Expression Regulation*
  • Genomic Imprinting*
  • Genomic Library
  • Insulin-Like Growth Factor II / genetics*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Promoter Regions, Genetic
  • Recombinant Proteins / metabolism
  • Repressor Proteins / metabolism*
  • Restriction Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection

Substances

  • GCFC2 protein, human
  • Recombinant Proteins
  • Repressor Proteins
  • Insulin-Like Growth Factor II