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Biochim Biophys Acta. 2001 Jun 15;1526(3):286-92.

Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus.

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Laboratory of Visual Information Processing, Institute of Biophysics, Baiao Pharmaceuticals Beijing C.L., Chinese Academy of Sciences, 15 Da Tun Rd, Chaoyang District, 100101, Beijing, PR China.


In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10-15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Physiol. Scand. 126 (1986) 601-607]. The enzyme could be detected in the intestinal epithelial cells by immunohistochemistry. Furthermore, immunoreactive intact EFE-III-1 was found in serum or plasma after intraperitoneal injection of rats. Approx. 10% of the full-size enzyme could transport through the intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection.

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