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Early Hum Dev. 2001 Aug;64(1):21-5.

Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a multiplexed microsphere-based immunoassay.

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Division of Genetic Disorders, Wadsworth Center, New York State Department of Health, 12201-0509, Albany, NY, USA.


We developed a fluorescent immunoassay to simultaneously measure antibodies to three HIV-1 antigens from newborn dried blood-spot specimens. The multiplexed assay uses fluorescent microspheres and a flow analyzer. The procedure is sensitive, precise and accurate, and can be expanded to simultaneously measure additional multiple analytes from a single specimen.

[Indexed for MEDLINE]

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