Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Chim Acta. 2001 Jul 5;309(1):37-43.

Expression of cathepsin L and its endogenous inhibitors in immortal and transformed fibroblasts.

Author information

1
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences (RAMS), Pogodinskaya St. 10, 119992, Moscow, Russia.

Abstract

METHODS:

The activities of cathepsin L and its endogenous inhibitors were analyzed in rat embryo fibroblasts, immortalized and transformed by different genes.

RESULTS:

Regardless of the transfecting agent used (DNA of adenovirus SA7 or polyomavirus LT gene), the immortal cells showed an increase in the cathepsin L activity (in both lysates and conditioned media) compared to primary fibroblasts. Transformed cells exhibited either an increase (with c-Ha-ras gene) or decrease (with E7 HPV gene) in cathepsin L activity in lysates as opposed to immortal cells.

CONCLUSIONS:

The data are suggestive of alterations in the trafficking of cathepsin L upon fibroblast transfection with polyomavirus LT gene and E7 HPV gene. An endogenous inhibitor(s) of cysteine proteinase was found in conditioned media, but not in lysates, of all cell cultures studied and its activity in normal fibroblasts was higher than in media of immortal and transformed cells.

PMID:
11408004
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center