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Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7449-54. Epub 2001 Jun 12.

Altered lymphocyte responses and cytokine production in mice deficient in the X-linked lymphoproliferative disease gene SH2D1A/DSHP/SAP.

Author information

1
National Human Genome Research Institute, National Cancer Institute, and National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

We have introduced a targeted mutation in SH2D1A/DSHP/SAP, the gene responsible for the human genetic disorder X-linked lymphoproliferative disease (XLP). SLAM-associated protein (SAP)-deficient mice had normal lymphocyte development, but on challenge with infectious agents, recapitulated features of XLP. Infection of SAP- mice with lymphocyte choriomeningitis virus (LCMV) or Toxoplasma gondii was associated with increased T cell activation and IFN-gamma production, as well as a reduction of Ig-secreting cells. Anti-CD3-stimulated splenocytes from uninfected SAP- mice produced increased IFN-gamma and decreased IL-4, findings supported by decreased serum IgE levels in vivo. The Th1 skewing of these animals suggests that cytokine misregulation may contribute to phenotypes associated with mutation of SH2D1A/SAP.

PMID:
11404475
PMCID:
PMC34689
DOI:
10.1073/pnas.131193098
[Indexed for MEDLINE]
Free PMC Article

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