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Syst Appl Microbiol. 2001 Apr;24(1):108-15.

Subtraction hybridization in microplates: an improved method to generate strain-specific PCR primers.

Author information

1
Lehrstuhl für Mikrobiologie, Technische Universität München-Weihenstephan, Freising, Germany.

Abstract

An improved subtraction hybridization technique was developed and evaluated. The hybridization is performed in a microplate with the subtractor-DNA immobilized in the plate while the probe-DNA is in solution. After hybridization the probe-specific DNA can easily be removed from the microwell and submitted to further analysis. This new technique has been successfully applied to generate several strain-specific PCR-primers for Lactococcus lactis subsp. lactis, Pediococcus spec., Saccharomyces spec. and Listeria monocytogenes.

PMID:
11403389
DOI:
10.1078/0723-2020-00014
[Indexed for MEDLINE]

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