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Biochem Biophys Res Commun. 2001 Jun 8;284(2):531-5.

Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry.

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Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62250, México.


Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+]i) it induces in sperm. We observed significant time delays for the increase in pH(i) and [Ca2+]i induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pH(i)-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the time sequence of the signaling events.

[Indexed for MEDLINE]

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