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Can J Physiol Pharmacol. 1975 Apr;53(2):317-20.

Phosphate uptake into organic compounds in skeletal muscle.


Isolated intact frog muscles were incubated in 32-P-labelled Ringer's solution for various periods of time (30 s-20 h). Labelled compounds were isolated from TCA, methanol-chloroform-water, and water extracts of muscle. Hexosephosphates, phosphocreatine, phosphoenolphyruvate, alpha-glycerol phosphate, adenosine triphosphate, and inorganic phosphate were identified after 30 s, and 4 h incubation. Much more labelling was found after 20 h. The incorporation of 32-P in 30 s into organic phosphate compounds, such as alpha-glycerol phosphate and ATP, showed that immediate esterification of Pi occured on, or just inside, the sarcolemma.

[Indexed for MEDLINE]

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