Fiber-optic fluorometer for microscale mapping of photosynthetic pigments in microbial communities

Appl Environ Microbiol. 2001 Jun;67(6):2823-8. doi: 10.1128/AEM.67.6.2823-2828.2001.

Abstract

Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 microm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We present details of the measuring system and describe examples of applications in different microbial communities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / analysis
  • Bacteriochlorophylls / analysis
  • Biofilms
  • Chlorophyll / analysis
  • Chlorophyta
  • Cyanobacteria
  • Ecosystem*
  • Fiber Optic Technology*
  • Fluorometry / instrumentation*
  • Geologic Sediments / microbiology
  • Light-Harvesting Protein Complexes
  • Marine Biology
  • Microbiology / instrumentation*
  • Optical Fibers
  • Photosynthesis*
  • Plant Proteins / analysis

Substances

  • Bacterial Proteins
  • Bacteriochlorophylls
  • Light-Harvesting Protein Complexes
  • Plant Proteins
  • Chlorophyll