Improvement of quantitative PCR reproducibility by betaine as determined by fluorescence-based method

Biotechniques. 2001 May;30(5):950-2, 954. doi: 10.2144/01305bm05.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Betaine*
  • Chromosome Breakage / genetics
  • Chromosomes, Human, Pair 22
  • DNA, Complementary / analysis
  • Dimethyl Sulfoxide
  • Electrophoresis, Agar Gel
  • Fluorescent Dyes*
  • Humans
  • Indicators and Reagents
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Taq Polymerase

Substances

  • DNA, Complementary
  • Fluorescent Dyes
  • Indicators and Reagents
  • Betaine
  • Taq Polymerase
  • Dimethyl Sulfoxide