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Proc Natl Acad Sci U S A. 2001 May 22;98(11):6360-5. Epub 2001 May 15.

CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A.

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  • 1The Picower Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA.

Abstract

Cyclophilin A (CyPA) is specifically incorporated into the virions of HIV-1 and has been shown to enhance significantly an early step of cellular HIV-1 infection. Our preliminary studies implicated CD147 as a receptor for extracellular CyPA. Here, we demonstrate a role for CyPA-CD147 interaction during the early steps of HIV-1 infection. Expression of human CD147 increased infection by HIV-1 under one-cycle conditions. However, susceptibility to infection by viruses lacking CyPA (simian immunodeficiency virus or HIV-1 produced in the presence of cyclosporin A) was unaffected by CD147. Virus-associated CyPA coimmunoprecipitated with CD147 from infected cells. Antibody to CD147 inhibited HIV-1 entry as evidenced by the delay in translocation of the HIV-1 core proteins from the membrane and inhibition of viral reverse transcription. Viruses whose replication did not require CyPA (SIV or mutant HIV-1) were resistant to the inhibitory effect of anti-CD147 antibody. These results suggest that HIV-1 entry depends on an interaction between virus-associated CyPA and CD147 on a target cell.

PMID:
11353871
PMCID:
PMC33473
DOI:
10.1073/pnas.111583198
[PubMed - indexed for MEDLINE]
Free PMC Article
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