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Genomics. 2001 May 1;73(3):272-83.

Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10).

Author information

  • 1The Center for Hearing Sciences, Johns Hopkins University, Baltimore, Maryland 21205, USA. llustig@bme.jhu.edu

Abstract

We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, alpha10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat alpha10. The alpha10 gene was localized to chromosome 11p15.5. Human alpha10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR alpha9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.

PMID:
11350119
DOI:
10.1006/geno.2000.6503
[PubMed - indexed for MEDLINE]
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