Format

Send to

Choose Destination
See comment in PubMed Commons below
J Virol Methods. 2001 May;94(1-2):1-6.

A novel retroviral vector that allows the magnetic selection of infected cells.

Author information

  • 1Department of Microbiology and Immunology, Markey Cancer Center, University of Kentucky, Lexington, KY40536-0096, USA. jzhanl@pop.uky.edu

Abstract

Retroviral vectors are used widely in research and are also being designed for use in gene therapy trials. In practice, these vectors usually contain a marker gene, which is often a drug selection gene. In this report, a novel retroviral vector has been constructed which contains a gene that allows selection for infected cells by a magnet. This gene is a single-chain antibody (sFv) to a specific hapten molecule 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (phOx). sFv specific for phOx is displayed on the surface of infected cells. This feature allows binding to phOx-BSA coated magnetic beads which are used to isolate the infected cells.

PMID:
11337034
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center