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Electrophoresis. 2001 Mar;22(5):843-54.

Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis.

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  • 1Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. jcs@bnl.gov

Abstract

DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.

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