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Immunopharmacol Immunotoxicol. 2001 Feb;23(1):97-106.

Inhibitory effect of tumor necrosis factor-alpha secretion from rat astrocytes by Chilbokeum.

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  • 1Department of Oriental Pharmacy, College of Pharmacy, Center of Oriental Medicinal Science, Wonkwang University, Iksan, South Korea.

Abstract

Substance P (SP) can stimulate secretion of tumor necrosis factor-alpha (TNF-alpha) from astrocytes stimulated with lipopolysaccharide (LPS). In this study, we have examined whether an aqueous extract of Chilbokeum inhibits secretion of TNF-alpha from primary cultures of rat astrocytes. Chilbokeum (10 microg/ml) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by Chilbokeum. Treatment of Chilbokeum (10 and 100 microg/ml) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Our results suggest that Chilbokeum may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that Chilbokeum has an antiinflammatory activity in the central nervous system.

PMID:
11322653
DOI:
10.1081/IPH-100102571
[PubMed - indexed for MEDLINE]
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