Mutations in the csgD promoter associated with variations in curli expression in certain strains of Escherichia coli O157:H7

G A Uhlich; J E Keen; R O Elder

doi:10.1128/AEM.67.5.2367-2370.2001

Mutations in the csgD promoter associated with variations in curli expression in certain strains of Escherichia coli O157:H7

Appl Environ Microbiol. 2001 May;67(5):2367-70. doi: 10.1128/AEM.67.5.2367-2370.2001.

Authors

G A Uhlich¹, J E Keen, R O Elder

Affiliation

¹ Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933, USA. guhlich@arserrc.gov

Abstract

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Base Sequence
Escherichia coli Infections / microbiology
Escherichia coli O157 / genetics*
Escherichia coli O157 / growth & development
Escherichia coli O157 / metabolism
Escherichia coli Proteins*
Genetic Variation
Humans
Mutation*
Promoter Regions, Genetic / genetics*
Sequence Analysis, DNA

Substances

Bacterial Proteins
CsgB protein, E coli
Escherichia coli Proteins
csgA protein, E coli
Crl protein, Bacteria