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Biol Trace Elem Res. 2000 Winter;78(1-3):67-79.

Method developments for iodine speciation by reversed-phase liquid chromatography-ICP-mass spectrometry.

Author information

1
GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany.

Abstract

Method developments are described for a iodine speciation method. This method is based on a reversed-phase liquid chromatography separation combined with inductively coupled plasma-mass spectrometry detection. Investigations are performed concerning the methanol tolerance/stability of inductively coupled plasma, postcolumn oxidation/volatilization of iodine from species, influences of buffer pH, and column temperature on separation efficiency. The experiments focused on six iodine species, namely iodine, monoiodothyrosine, di-iodothyrosine, tri-iodothyronine, reversed tri-iodothyronine, and thyroxin. Detection limits were determined between 0.08 and 1.5 microg/L (iodine related), dependent on species. Appplications on a urine sample were performed, showing iodide as the predominant iodine species.

PMID:
11314989
DOI:
10.1385/BTER:78:1-3:67
[Indexed for MEDLINE]

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