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Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5128-33. Epub 2001 Apr 17.

An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY.

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1
Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.

Abstract

The SecY/Sec61alpha family of membrane proteins are the central subunits of the putative protein translocation channel. We introduced random mutations into a segment of Escherichia coli SecY within its cytoplasmic domain 5, which was shown previously to be important for the SecA-dependent translocation activity. Mutations were classified into those retaining function and those gaining a dominant-interfering ability caused by a loss of function. These analyses showed that Arg-357, Pro-358, Gly-359, and Thr-362 are functionally important; Arg-357, conserved in almost all organisms, was identified as an indispensable residue.

PMID:
11309495
PMCID:
PMC33175
DOI:
10.1073/pnas.081617398
[Indexed for MEDLINE]
Free PMC Article
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