Abstract
Here we describe the isolation of the zebrafish fgfr3 gene, its structure and chromosomal location. Expression in wild type embryos occurs in the axial mesoderm, the diencephalon, the anterior hindbrain and the anterior spinal cord. In the hindbrain, a differential expression of fgfr3 was detected at several levels of intensity, with the highest expression in the posterior rhombomere 1 that is morphologically distinct from the anterior part, which develops into the cerebellum. Further, analysis of fgfr3 expression in mutants deficient in the formation of the midbrain-hindbrain boundary (MHB), noi(-/-) and ace(-/-), demonstrated that in the absence of Pax2.1 and FGF8 activity, the expression domains of FGFR3 expand into the MHB, tegmentum, cerebellum and optic tectum, which are the affected structures in these mutants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Brain / embryology
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Brain / metabolism
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Cerebellum / embryology
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Cerebellum / metabolism
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Chromosome Mapping
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DNA-Binding Proteins / biosynthesis
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors / biosynthesis
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In Situ Hybridization
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Models, Genetic
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Molecular Sequence Data
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Mutation
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Neural Crest / embryology*
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Neural Crest / metabolism*
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PAX2 Transcription Factor
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Protein Structure, Tertiary
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Protein-Tyrosine Kinases*
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Receptor, Fibroblast Growth Factor, Type 3
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Receptors, Fibroblast Growth Factor / biosynthesis*
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Receptors, Fibroblast Growth Factor / genetics*
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Spinal Cord / embryology
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Spinal Cord / metabolism
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Superior Colliculi / metabolism
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Tissue Distribution
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Transcription Factors / biosynthesis
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Zebrafish
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Zebrafish Proteins
Substances
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DNA-Binding Proteins
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PAX2 Transcription Factor
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Receptors, Fibroblast Growth Factor
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Transcription Factors
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Zebrafish Proteins
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pax2a protein, zebrafish
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors
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Protein-Tyrosine Kinases
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Receptor, Fibroblast Growth Factor, Type 3
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fgfr3 protein, zebrafish