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Carbohydr Res. 2001 Mar 9;331(1):101-6.

A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa -carrageenase.

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  • 1Institute of Chemistry and Biotechnology, Agricultural University of Norway, As.


Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosaccharides. It was used to evaluate the elution profiles of hydrolysates produced by carrageenases specific for kappa- and iota-carrageenans. Oligosaccharide peaks ranging from di- to dodeca-saccharides were obtained in about 20 min on an analytical scale, whereas preparative runs were completed in a few hours. The method may also be used to monitor polysaccharide degradation.

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