Small volume bead assay for ovalbumin with electrochemical detection

Analyst. 2001 Mar;126(3):337-41. doi: 10.1039/b006798i.

Abstract

A bead based sandwich enzyme immunoassay coupled to electrochemical detection for ovalbumin has been developed. The enzyme label alkaline phosphatase was used to convert the substrate 4-aminophenyl phosphate to electroactive product 4-aminophenol. The detection was done in a microdrop by continuously monitoring the enzyme turnover with a rotating disk electrode. This reduces dilution of the enzyme product, a key to achieving low detection limits. The assay developed has a detection limit of 0.1 ng ml-1. Assay sensitivity in complex matrices such as food and serum was compared.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Electrochemistry
  • Excipients / analysis*
  • Food Analysis / methods*
  • Ice Cream / analysis*
  • Immunoenzyme Techniques
  • Microchemistry
  • Ovalbumin / analysis*
  • Ovalbumin / blood
  • Rats
  • Sensitivity and Specificity

Substances

  • Excipients
  • Ovalbumin