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Nat Biotechnol. 2001 Apr;19(4):365-70.

Single-mismatch detection using gold-quenched fluorescent oligonucleotides.

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1
Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. benoit@pollux.rockefeller.edu

Erratum in

  • Nat Biotechnol 2001 Jul;19(7):680-1.

Abstract

Here we describe a hybrid material composed of a single-stranded DNA (ssDNA) molecule, a 1.4 nm diameter gold nanoparticle, and a fluorophore that is highly quenched by the nanoparticle through a distance-dependent process. The fluorescence of this hybrid molecule increases by a factor of as much as several thousand as it binds to a complementary ssDNA. We show that this composite molecule is a different type of molecular beacon with a sensitivity enhanced up to 100-fold. In competitive hybridization assays, the ability to detect single mismatch is eightfold greater with this probe than with other molecular beacons.

PMID:
11283596
DOI:
10.1038/86762
[Indexed for MEDLINE]
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