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Virology. 2001 Mar 15;281(2):294-304.

An infectious clone of the West Nile flavivirus.

Author information

1
Department of Internal Medicine, University of Virginia, Charlottesville, Virginia 22908, USA. vfy9e@virginia.edu

Abstract

West Nile (WN) virus is the most widespread among flaviviruses, but until recently it was not known on the American continent. We describe here design of a subgenomic replicon, as well as a full-length infectious clone of the lineage II WN strain, which appeared surprisingly stable compared to other flavivirus infectious clones. This infectious clone was used to investigate effects of 5'- and 3'-nonrelated sequences on virus replication and infectivity of synthetic RNA. While a long nonrelated sequence at the 3'-end delayed but did not prevent establishment of the productive infectious cycle, a much shorter extra sequence at the 5'-end completely abrogated virus replication. Replacement of the conserved 5'-adenosine residue substantially delayed, but did not prevent, establishment of virus infection. In all cases, the recovered virus had restored its authentic 5'- and 3'-end genome sequences. However, the presence of extensive nonrelated sequences at both 5'- and 3'-ends could not be repaired.

PMID:
11277701
DOI:
10.1006/viro.2000.0795
[Indexed for MEDLINE]
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