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Med Mycol. 2001 Feb;39(1):103-7.

The use of flow cytometry as a tool for monitoring filament formation of fungi.

Author information

1
Clinical Microbiology and Immunology Laboratories UNC Hospitals, Chapel Hill, NC 27514, USA. rhopfer@unch.unc.edu

Abstract

Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.

PMID:
11270396
DOI:
10.1080/mmy.39.1.103.107
[Indexed for MEDLINE]

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