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FEMS Microbiol Lett. 2001 Mar 15;196(2):251-6.

Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus.

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1
Department of Pharmacology, School of Dentistry, Iwate Medical University, 020-8505, Morioka, Japan.

Abstract

We have cloned and sequenced the gene encoding the surface protein antigen PAa (antigen I/II family) from Streptococcus cricetus E49 (serotype a) using degenerate PCR. The deduced amino acid sequence of PAa reveals two repeating regions (A region; alanine-rich region, P region; proline-rich region). Two additional tandem repeats were found in the A region and part of the P region was deleted compared to antigen I/II. Homology and phylogenetic analyses reveal that PAa is homologous to Streptococcus sobrinus PAg rather than Streptococcus mutans PAc. Using degenerate PCR a gene homologous to PAc was identified in Streptococcus intermedius, but not found in Streptococcus rattus or Streptococcus anginosus.

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