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EMBO Rep. 2001 Feb;2(2):119-23.

MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3.

Author information

1
Wellcome/CRC Institute, Cambridge, UK. yt215@cam.ac.uk

Abstract

The MCM proteins are essential for the initiation of DNA replication. We have isolated an MCM3-associated protein (MCM3AP) in a two-hybrid screen using MCM3. Here we demonstrate that MCM3AP is an acetyltransferase which acetylates MCM3 and that chromatin-bound MCM3 is acetylated in vivo. The MCM3 acetylase, MCM3AP, is also chromatin-bound. This study also indicates that MCM3AP contains putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily. Mutation of those motifs significantly inhibits the MCM3 acetylase activity. Over-expression of MCM3AP inhibits DNA replication, whereas mutation of the acetylase motifs abolishes this effect, suggesting that acetylation plays a role in DNA replication. Taken together, we suggest that MCM3 acetylation is a novel pathway which might regulate DNA replication.

PMID:
11258703
PMCID:
PMC1083822
DOI:
10.1093/embo-reports/kve026
[Indexed for MEDLINE]
Free PMC Article

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