Abstract
The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in L. lactis and Escherichia coli, but not in Enterococcus faecalis or in Staphylococcus aureus. Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in L. lactis and in E. coli.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology*
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Base Sequence
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Carrier Proteins / genetics*
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Carrier Proteins / physiology*
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Cloning, Molecular
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Drug Resistance, Microbial*
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Drug Resistance, Multiple*
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Erythromycin / metabolism
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Escherichia coli / drug effects*
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Genes, Bacterial
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Kinetics
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Lactococcus lactis / drug effects*
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Lactococcus lactis / genetics
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Lactococcus lactis / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / physiology*
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Membrane Transport Proteins*
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Molecular Sequence Data
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Phenotype
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Tetracycline / metabolism
Substances
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Bacterial Proteins
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Carrier Proteins
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Escherichia coli Proteins
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Mdt-A protein, E coli
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Membrane Proteins
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Membrane Transport Proteins
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Erythromycin
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Tetracycline