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Gene. 2001 Feb 21;264(2):225-31.

Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein.

Author information

1
Department of Medicine, Division of Infectious Diseases, University of British Columbia, 2733 Heather St., Vancouver, British Columbia, Canada V5Z 3J5.

Abstract

Two green fluorescent protein (Gfp) fusion vectors were constructed for use in Mycobacterium spp. The first plasmid facilitates quantification of mycobacterial promoter activity. The second vector permits construction of translational fusions of mycobacterial proteins to Gfp in order to study subcellular localization including protein secretion. Using this translational fusion construct, we verify that a Gfp fusion to the putative secreted M. tuberculosis protein ChoD is translocated to the extracellular milieu when cloned and expressed in Mycobacterium smegmatis.

PMID:
11250077
DOI:
10.1016/s0378-1119(01)00336-5
[Indexed for MEDLINE]

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