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Gene. 2001 Feb 21;264(2):173-85.

Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana.

Author information

1
Dipartimento di Genetica e Biologia dei Microrganismi, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.

Abstract

NF-Y is a CCAAT-specific binding factor composed of three distinct subunits. In vertebrates and fungi all three subunits are encoded by evolutionary conserved single copy genes. In this report we have cloned twenty-three NF-Y genes in A. thaliana, assessed their mRNA expression levels in a large number of tissues and confirmed that indeed multiple CCAAT-binding activities are present. Alignments of the genes coding for the three NF-Y subunits yield a considerable amount of information concerning the divergence/conservation of protein subdomains and of single residues within the conserved parts. Careful evaluation of mRNA expression levels by sensitive RT-PCR assays provide evidence that all three subunits have members that are ubiquitous and others that are tissue-specific and induced only after the switch to reproductive growth phase, in flowers and siliques.

PMID:
11250072
DOI:
10.1016/s0378-1119(01)00323-7
[Indexed for MEDLINE]

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