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Inflamm Res. 2001 Jan;50(1):1-5.

Induction of IL-6 via the EP3 subtype of prostaglandin E receptor in rat adjuvant-arthritic synovial cells.

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1
Department of Internal Medicine, Kitasato University, School of Medicine, Kanagawa , Japan.

Abstract

OBJECTIVE AND DESIGN:

This study investigated the role of the prostaglandin E receptor EP3 subtype in the development of inflammatory synovitis.

MATERIALS AND METHODS:

We examined the EP3 mRNA expression in the synovial tissue of rats with adjuvant arthritis and the effect of selective EP agonists on IL-6 production by cultured rat synovial cells.

RESULTS:

Of the two rat EP3 isoforms, rEP3B mRNA but not rEP3A mRNA was expressed in rat synovial cells. RT-PCR and ELISA were used to analyze the effects of selective EP3 agonist (ONO-AE-248) on IL-6 production by rat cultured synovial cells. This selective EP3 agonist induced IL-6 production by rat synovial cells in a time- and dose-dependent manner.

CONCLUSIONS:

Our results suggest that PGE2 upregulates IL-6 production by synovial macrophages and fibroblasts through rEP3B in rats with adjuvant arthritis.

PMID:
11235015
DOI:
10.1007/s000110050716
[Indexed for MEDLINE]
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