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FEBS Lett. 2001 Jan 26;489(1):25-8.

A suicide-substrate mechanism for hydrolysis of beta-lactams by an anti-idiotypic catalytic antibody.

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Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, P.O. Box 20529, 60205 Cedex, Compiegne, France.


The catalytic mechanism of an anti-idiotypic antibody, 9G4H9, displaying a beta-lactamase activity was investigated. Kinetics experiments suggest that some penicillinic derivatives behave both as substrates and inactivators. Biochemical and immunological experiments strongly indicate that ampicillin may be regarded as a suicide substrate for hydrolysis by 9G4H9. The anti-idiotypic network appears as a way to create enzyme mimics with modified catalytic activities.

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