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J Chromatogr A. 2001 Jan 12;907(1-2):145-54.

Membrane ion-exchange chromatography for process-scale antibody purification.

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Department of Recovery Sciences, Genentech, Inc., South San Francisco, CA 94080, USA.


The large-scale production of recombinant monoclonal antibodies demands economical purification processes with high throughputs. The potential for ion-exchange membrane adsorbers to replace traditional ion-exchange columns was evaluated. Breakthrough capacities of commercially available cation-exchange membranes were determined as a function of flow-rate and layer number. Due to economic and process restrictions, cation-exchange membranes may not currently be advantageous for process-scale antibody purification in a bind and elute mode. However, anion-exchange membranes in a flow-through mode may provide a reasonable alternative to columns for the removal of low levels of impurities such as DNA, host cell protein, and virus.

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