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Anticancer Res. 2000 Nov-Dec;20(6B):4147-51.

TRAG-3, a novel cancer/testis antigen, is overexpressed in the majority of melanoma cell lines and malignant melanoma.

Author information

1
Division of Hematology/Oncology, Massachusetts General Hospital, Fruit Street, Boston, MA 02114, USA.

Abstract

BACKGROUND:

We have identified a novel cancer/testis antigen, TRAG-3, (Taxol Resistance Associated Gene-3) that was initially discovered in search for new genes involved in drug resistance by differential display. Early study of TRAG-3 revealed minimal to absent expression in various normal tissues and over-expression in many carcinoma cell lines including several melanoma lines.

MATERIALS AND METHODS:

Northern and RT-PCR technologies were used to evaluate TRAG-3 expression in numerous cell lines and tumor tissue.

RESULTS:

Analysis of a wider panel of normal tissues, 32 melanoma cell lines and 4 malignant melanomas demonstrates TRAG-3 expression in 25 of the 32 melanoma cell lines (78%) and four of four of the malignant melanoma tumors (100%). Of the additional eight normal tissues screened, expression was present in normal testis but absent in all other tissues. RT-PCR evaluation of TRAG-3 reveals two transcripts in many carcinoma cell lines with sequencing of these products demonstrating the 799 bp TRAG-3 transcript and a second alternatively spliced transcript, TRAG-3long TRAG-3 maps to band Xq28 within a MAGE gene complex, however sequence analysis demonstrates that TRAG-3 is not homologous to other known cancer/testis antigens.

CONCLUSION:

TRAG-3 appears to be a novel cancer/testis antigen.

PMID:
11205240
[Indexed for MEDLINE]

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