Format

Send to

Choose Destination
Am J Vet Res. 2001 Jan;62(1):33-6.

Evaluation of the cardiac actin gene in Doberman Pinschers with dilated cardiomyopathy.

Author information

1
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus 43026, USA.

Abstract

OBJECTIVE:

To evaluate the coding region of the cardiac actin gene in Doberman Pinschers with dilated cardiomyopathy (DCM) for mutations that could be responsible for the development of the condition

ANIMALS:

28 dogs (16 Doberman Pinschers with DCM and 12 mixed-breed control dogs).

PROCEDURE:

Ten milliliters of blood was collected from each dog for DNA extraction. Polymerase chain reaction (PCR) primers were designed to amplify canine exonic regions, using the sequences of exons 2 to 6 of the cardiac actin gene. Single-stranded conformational polymorphism analysis was performed for each exon with all samples. Autoradiographs were analyzed for banding patterns specific to affected dogs. The DNA sequencing was performed on a selected group of affected and control dogs.

RESULTS:

Molecular analysis of exons 2 to 6 of the cardiac actin gene did not reveal any differences in base pairs between affected dogs and control dogs selected for DNA evaluation.

CONCLUSIONS:

Mutations in exons 5 and 6 of the cardiac actin gene that have been reported in humans with familial DCM do not appear to be the cause of familial DCM in Doberman Pinschers. Additionally, evaluation of exons 2 to 6 for causative mutations did not reveal a cause for inherited DCM in these Doberman Pinschers. Although there is evidence that DCM in Doberman Pinschers is an inherited problem, a molecular basis for this condition remains unresolved. Evaluation of other genes coding for cytoskeletal proteins is warranted.

PMID:
11197556
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center