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Am J Vet Res. 2001 Jan;62(1):29-32.

Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.

Author information

1
Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504, USA.

Abstract

OBJECTIVE:

To develop and evaluate a polyvalent ELISA incorporating a highly specific recombinant antigen (p44) for diagnosis of granulocytic ehrlichiosis in dogs and horses.

ANIMALS:

32 dogs and 43 horses.

PROCEDURE:

Results of the ELISA were compared with results of indirect fluorescent antibody (IFA) staining and western immunoblotting incorporating whole-cell antigen.

RESULTS:

For the canine and equine samples, percentages of samples with positive IFA staining, western immunoblotting, and ELISA results were similar. For 29 (91 %) canine samples and 30 (70%) equine samples, results of IFA staining, western immunoblotting, and the ELISA were in complete agreement. Results of the ELISA for 3 canine serum samples known to contain antibodies to Ehrlichia canis and 12 equine serum samples known to contain antibodies to E risticii were negative.

CONCLUSIONS AND CLINICAL RELEVANCE:

Results of the present study suggest that a polyvalent ELISA incorporating a recombinant p44 antigen is suitable for detecting antibodies to E equi in dogs and horses.

PMID:
11197555
DOI:
10.2460/ajvr.2001.62.29
[Indexed for MEDLINE]

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