Format

Send to

Choose Destination
Mol Plant Microbe Interact. 2001 Jan;14(1):98-103.

Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance.

Author information

1
Department of Crop Sciences, University of Illinois at Urbana-Champaign, 61801, USA.

Abstract

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.

PMID:
11194879
DOI:
10.1094/MPMI.2001.14.1.98
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center