Abstract
A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Babesia / classification
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Babesia / genetics
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Babesia / isolation & purification*
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Babesia bovis / genetics
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Babesia bovis / isolation & purification
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Babesiosis / blood
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Babesiosis / diagnosis
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Babesiosis / veterinary*
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Cattle
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Cattle Diseases / blood
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Cattle Diseases / diagnosis*
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DNA, Ribosomal / genetics
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Italy
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Luminescent Measurements
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Nucleic Acid Hybridization
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Polymerase Chain Reaction / methods
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RNA, Protozoan / genetics
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RNA, Ribosomal, 18S / genetics
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Theileria / classification
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Theileria / genetics
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Theileria / isolation & purification*
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Theileria annulata / genetics
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Theileria annulata / isolation & purification
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Theileria parva / genetics
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Theileria parva / isolation & purification
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Theileriasis / blood
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Theileriasis / diagnosis*
Substances
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DNA, Ribosomal
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RNA, Protozoan
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RNA, Ribosomal, 18S