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J Mol Biol. 2001 Feb 23;306(3):403-16.

Anatomy of a preferred target site for the bacterial insertion sequence IS903.

Author information

1
Division of Infectious Disease, Wadsworth Center, New York State Dept. of Health, Albany, NY 12201, USA.

Abstract

Like many transposons the bacterial insertion sequence IS903 was thought to insert randomly. However, using both genetic and statistical approaches, we have derived a target site for IS903 that is used 84% of the time. Computational and genetic analyses of multiple IS903 insertion sites predicted a preferred target consisting of a 21 bp palindromic pattern centered on the 9 bp target duplication generated during transposition. Here we show that targeting can be dissected into four components: the 5 bp flanking sequences, the most important sequences required for site-specific insertion; the 7 bp palindromic core within the target duplication; the dinucleotide pair at the transposon-target junction; and the local DNA context. Finally, using a substrate with multiple target sites we show that a target site is more likely found by a local bind-and-slide model and not by extended DNA tracking.

PMID:
11178901
DOI:
10.1006/jmbi.2000.4421
[Indexed for MEDLINE]

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