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J Appl Microbiol. 2001 Feb;90(2):229-36.

A colony blot immunoassay to detect enteroinvasive Escherichia coli and Shigella in water samples.

Author information

1
Department of Medical Microbiology and Immunology, Pécs University, Faculty of Medicine, H-7643 Pécs, Szigeti út 12, Hungary.

Abstract

AIMS:

The aim of the study was to develop a colony blot immunoassay to detect Shigella and enteroinvasive Escherichia coli (EIEC) in water.

METHODS AND RESULTS:

Spiked samples were filtered through nitrocellulose membranes. Colony prints on the filters were tested with a monoclonal antibody specific to IpaC, an antigen coded by the invasion plasmid of Shigella and EIEC. Invasive pathogens could be successfully detected with the technique, even in the presence of a large number of non-pathogenic bacterial cells. The method was significantly more sensitive in identifying pathogen-containing samples then the traditional culture-based approach.

CONCLUSION:

The IpaC-specific colony blot immunoassay is an inexpensive method for identifying the aetiological agents of bacillary dysentery in water samples.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The technique could be particularly useful in detecting enteroinvasive E. coli which often remains undetected by bio- and serotyping.

[Indexed for MEDLINE]
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